Hidden threat lurking in extensive hand hygiene during the Covid-19 pandemic: investigation of sensitizing molecules in gel products by hyphenated chromatography techniques.

During the Covid-19 pandemic, health agencies worldwide have recommended frequent handwashing and sanitizing. A variety of hand gel products were made available on the market, often with fragrances added to curtail the strong smell of alcohol. Commonly used Citrus fragrances contain volatile aroma constituents and non-volatile oxygen heterocyclic compounds (OHCs), consisting mostly of polymethoxyflavones, coumarins, and furocoumarins. The latter have long been investigated for their phototoxic properties, and their safety as cosmetic product ingredients has been debated recurrently. To this concern, twelve commercial Citrus-scented products were investigated in this study. An extraction method was optimized for thirty-seven OHC compounds, obtaining absolute mean recovery values in the 73.5-116% range with only few milliliters of solvent consumption. Analysis by ultra-high-pressure liquid chromatography with tandem mass spectrometry detection evidenced that three samples did not conform to the labeling requirements for fragrance allergens (coumarin) laid down by the European Union Regulation on Cosmetic Products. The total furocoumarin (FC) content of the samples investigated was in the 0.003-3.7ppm range, with some noteworthy exceptions. Specifically, in two samples, the total FCs were quantified as 89 and 219 ppm, thus exceeding the safe limits recommended up to a factor of 15. Finally, the consistency of the volatile fingerprint attained by gas chromatography allowed drawing conclusions on the authenticity of the Citrus fragrances labeled, and several products did not conform to the information reported on the label concerning the presence of essential oils. Besides the issue of product authenticity, analytical tools and regulatory actions for widespread testing of hand hygiene products are urgent, to protect consumers' health and safety.

Review on Naringin: Method of Isolation, Analytical Development, and Its Recent Pharmacological Activities

Naringin is a flavonoid isolated from different citrus fruits like Grapefruit, Orange, Pomelo, Lemon, etc, which are commonly called local fruit. Naringin is found in the white spongy portion of citrus peel. Its content varies from 0.65 mg/gm in the mandarin peel to 14.40mg/gm in the grapefruit peel. Naringin is metabolized to the flavanone naringenin by the enzyme Naringinase present in the liver. It can be analyzed by using various analytical techniques such as HPLC, TLC, UV, HPTLC, mass spectroscopy, Liquid chromatography, chiral chromatography and LC/Mass spectroscopy. Naringin can act as an antioxidant and scavenge free radicals. Naringin mainly focuses on in-vitro and in-vivo animal studies showing its beneficial effects on cardioprotective, antioxidant, anti-inflammatory, antimicrobial, hypolipemiant, neurological, thermogenic, pulmonary disorders and antidiabetic. Naringin is also treated as a most promising treatment strategy against Covid-19 due to its antiviral and anti-inflammatory effects. Recently, Naringin has proven its activity in various molecular docking studies. Naringin keeps the body healthy against various illnesses and major lifestyle disorders.Copyright All © 2023 are reserved by International Journal of Pharmaceutical Sciences and Research.

Immunomodulatory Activity of the Aqueous Extract of the pith of Citrus limon L. (Rutaceae) Using Cyclophosphamide Induced Myelosuppression

The genus Citrus species have been widely used in the relief of arthritic pain in traditional medicine. Citrus limon pith was one of the constituents of herbal decoctions taking daily for prevention against Coronavirus disease during covid-19 era. This study investigated the immunomodulatory activity of the aqueous extract of the pith of C. limon using cyclophosphamide-induced myelosuppression and delayed type hypersensitivity reaction in Wistar rats. Thirty-six rats were divided into six groups of six animals each. Three groups served as the control while the remaining three groups were given 150, 300, 600 mg/kg of extract respectively via oral route for 13 days. Groups II to VI were administered cyclophosphamide 30 mg/kg i.p on day 11, 12, and 13. On the 14th day, blood samples were collected for haematological parameters analysis. Delayed type hypersensitivity reaction was carried out using 24 animals divided into four groups of six animals each. Phytochemical screening revealed the presence of triterpenoids, flavonoids, and carbohydrates. LD50 was found to be greater than 3000 mg/kg. C. limon extract on cyclophosphamide-induced myelosuppression showed a statistically significant (P<0.05) increase in the white blood cells (WBC), platelets and lymphocytes counts at all extract doses. In the delayed type of hypersensitivity reaction, percentage increase in inflammation was 24.3%, 31.5% and 43% at extract doses of 150, 300 and 600 mg/kg. The findings of this study have shown that the aqueous extract of the pith of C. limon possessed immunostimulatory activity. © 2023 Shorinwa and Otu and the authors.

Self-medication Practices for COVID-19 Prevention: A Study among Medical Students in Yogyakarta, Indonesia

The corona virus disease 2019 (COVID-19) pandemic has increased the interest in self-care strategies, including self-medication. Medical students, as future health practitioners, learn more about medications than other students. This study aimed to describe self-medication practices for preventing COVID-19 among medical students at Universitas Islam Indonesia. This observational study used a cross-sectional design and was undertaken in November- December 2020. The study sample included 336 undergraduate medical students determined using a consecutive sampling technique based on inclusion and exclusion criteria. Data were collected using an online questionnaire about self-medication practices in the preceding 3 months. Among a total of 336 students, 137 (41%) reported using self-medication intending to prevent COVID-19, and 126 (92%) of these 137 took preventive supplements, mainly vitamins C, D, and E, and omega-3. Seven students reported the use of zinc, mainly in combination with other vitamins. Students who practiced self-medication lived closer to people confirmed with COVID-19, washed their hands more often, and desinfected their belongings more frequently than their counterparts (p<0.05). The mean duration for consuming vitamins was 11-16 days. Forty students (29%) used herbal medicine to prevent COVID-19;ginger, turmeric, honey, black seed, cutcherry, and Curcuma were the most often consumed herbal remedies. Fourteen students (10%) reported taking over-the-counter medications to relieve symptoms related to COVID-19, including antipyretic, analgesic, antiseptic, antihistamine, decongestant, antitussive, and expectorant medications. Most respondents (82%) purchased their medications at pharmacies, and 11 (9%) obtained them from online shops. These findings show the high rate of self-medication using vitamins and herbal remedies for COVID-19 prevention among university medical students. Further studies are needed to explore students' knowledge about the risks of self-medication including the use of herbal medicines.Copyright © 2022 Marmara University Press.

Nanomedicine and Drug Delivery Systems: Roles, Advantages and Disadvantages

Nanotechnology is a new and rapidly evolving subject in the pharmacological and therapeutic professions. Nanoparticles have many advantages as medication delivery systems, including increased efficacy and fewer adverse drug reactions. This study investigated the roles of nanomedicine and drug delivery systems in the pharmaceutical industry, as well as the advantages and disadvantages of nanotechnology. The study used a qualitative research technique, with online survey questionnaires sent to medical professionals and experts in the field of nanomedicine. These surveys comprised open-ended questions that enabled respondents to record their responses in whatever way they deemed fit. The ten respondents were from a variety of medical and health institutes, as well as medical consulting firms. In terms of results, the research established that nanomedicine had been used in medical care for therapy and diagnostic purposes. They are being explored in clinical trials for several reasons. Nanoparticles are used to treat renal disease, Tuberculosis, skin problems, Alzheimer's disease, and various types of cancer and to create COVID-19 vaccines. Further information about the study findings may be found in the results and discussion chapter.Copyright © 2022 Dr. Yashwant Research Labs Pvt. Ltd.. All rights reserved.

Public perception of citrus fruit consumption during the COVID-19 pandemic in Brazil

Adequate nutrition has become a priority for the psychologically and emotionally affected population, especially those under stress due to quarantine and people affected by Coronavirus Disease in 2019 (COVID-19). However, records on eating behavior variations due to Brazil's pandemic are still incipient, and it is crucial to know and identify frequent incentives that arouse interest in a class of products. Therefore, this study aimed to analyze the final consumer behaviors and the consumption influence of citrus fruits and their products, as a vitamin C source, in the COVID-19 pandemic in Brazil. By applying an online questionnaire prepared on the Google.. Forms platform, a survey was conducted with the voluntary participation of 546 unidentified respondents. The answers obtained made it possible to trace the participants' sociodemographic characteristics and consumption habits. According to the interviewees, there was a predominance of adults between 25 and 34 years old, female, single, living in the Southeast macro-region, and people with a master's and doctorate. level education. With the onset of the COVID-19 pandemic, most of the population interviewed did not change their consumption of citrus fruits and their products, but the place of purchase changed, possibly due to social isolation. Marketing is of fundamental importance in the Brazilian citrus industry, which is facing the challenge of encouraging a healthier diet and searching for expansion of the fresh fruit market.

Identification of potential phytochemicals from Citrus Limon against main protease of SARS-CoV-2: molecular docking, molecular dynamic simulations and quantum computations.

The outbreak of coronavirus disease (COVID-19) caused by a novel RNA virus emerged at the end of 2019. Most of the patient's symptoms are mild to moderate, and influenza, acute respiratory distress syndrome (ARDS) and multi-organ failure are common. The disease is mild to moderate in most patients and is reported in many cases such as pneumonia, ARDS and multi-organ dysfunction. This study's objective is to evaluate 25 natural compounds from Citrus limon (CL) used by comprehensive molecular docking, density functional theory (DFT) and molecular dynamics analysis against SARS-CoV-2 main protease (Mpro). Among all the experimental compounds, diosmetin has shown the best docking values against the Mpro of SARS-CoV-2 compared to the standard antiviral drug. In DFT calculations, the order associated with biochemical reactivity is as follows: eriodictoyl > quercetin > spinacetin > diosmetin > luteolin > apigenin, whereas the regions of oxygen and hydrogen atoms from the selected isolated compounds are appropriate for electrophilic and nucleophilic attacks, respectively. Also, HOMO-LUMO and global descriptors values indicated a promising result of these compounds. Moreover, a molecular dynamics simulation study revealed the stable conformation and binding pattern in a stimulating environment of natural compounds CL. Considering molecular docking, simulation, and DFT analysis of the selected compounds, notably eriodictoyl, quercetin, and diosmetin showed good potential against SARS-CoV-2 Mpro. Our in silico study revealed promising antiviral activity, which may be considered a potential key factor or a therapeutic target for COVID-19.Communicated by Ramaswamy H. Sarma.

The dental office: A safe place against COVID19 and other future pandemics

Background: The pandemic caused by the spread of COVID19 generated throughout the world great changes in all areas of life. Social distancing was carried out very drastically in some countries, and even in the field of dental care, some countries prohibited the practice of dentistry. Objective(s): To demonstrate that the dental office is safe regarding the possibility of contagion of Sars-Cov-2 as long as good biosecurity protocols are used. Method(s): A structured survey of 19 questions was applied to 103 patients who subsequently attended a periodontics and oral and maxillofacial surgery office in Mexico, to receive care from these specialties between April 2020 and July 2021. The questions posed in this survey were aimed at knowing the opinion of the patients regarding the measures that this establishment has for the prevention of the transmission of COVID19, as well as questions regarding the possible effects of attending this office in contagion of the disease from patients and their families. Result(s): A total of 18 (17.5%) patients reported having suffered COVID19, and none reported that they fell ill after the consultation. None of the clinic's specialists and assistants have fallen ill with COVID-19 during the pandemic. Conclusion(s): It is not necessary to suspend or condition dental care during times of pandemics such as what happened with COVID19, as long as strict safety protocols are observed within clinical facilities.Copyright © 2022 The Authors

Evaluation of the black soybean hulls agro-industrial waste for chloroquine removal from aqueous medium and treatment of multi-components

The COVID-19 pandemic increased the pollution of water resources by some contaminants, e.g., chloroquine (CQN), due to its probable benefit in the treatment of the virus. Thus, is necessary the removal of CQN from water through advanced techniques. Black soybeans have been widely used due to their benefits to human health, and as a result, there was an increase in soybean husk residue, the main by-product of the soybean processing industry. Given the current scenario and the need to develop new uses for this agricultural residue, this study aimed to establish an economical and environmental biotechnology by the CQN adsorption process onto black soybean hulls (BSH) for the first time. BSH was characterized by physicochemical and spectroscopic techniques that demonstrated porosity, organic functional groups and negative surface charges. The pH study did not affect CQN adsorption pronouncedly, indicating that π-interactions and hydrogen bonds are the main mechanisms of the adsorption process. The maximum adsorption capacity was 75.06 ± 2.24 mg g−1 with 240 min of contact time at 288 K. In order to verify the biosorbent applicability, the safranin orange dye and triclosan adsorption were also evaluated onto BSH. The absorption peaks of the contaminants used in the synthetic mixture demonstrated a removal rate of 90.81 ± 0.80% for safranin orange, 66.79 ± 1.12% for triclosan and 70.62 ± 0.67% for CQN. The satisfactory removal of other contaminants indicates that BSH is a promising, affordable and environmentally friendly biosorbent with applicability potential for alternative treatment of contaminated water. © 2023 Taylor & Francis Group, LLC.

The rediscovery of characteristic rural landscapes. An opportunity to create new activities?

For some years now, researchers in many geographical and other disciplinary fields have believed that historical rural landscapes contain an educational value for the landscapes of the future, in terms of analysis as well as protection in general and geo-economic aspects in particular, as an identity expression of the territory and as a preponderant element for the recovery of the same. In fact, in landscape studies the idea is widespread that historical and characteristic rural landscapes are not only the elements of a cultural heritage capable of preserving the memory of the past but also the bearers of a rational perspective for the future of European agriculture. The COVID-19 pandemic caused social and economic changes;critical issues highlight the need for a more integrated approach, taking into account the rediscovery of rural landscapes to practice open-air activities. Through a review of the international literature and the analysis of case studies, the paper aims to highlight the renewal processes of some cultural practices abandoned over the years but which today are an integral part of the local economy. In particular, the paper analyses the cases of two characteristic citrus groves in Apulia and Sicily, in the south of Italy. They demonstrate how the renewal of ancient practices such as the cultivation of citrus fruits together with the value of landscapes can represent an opportunity for an economic development of territories. © 2022 Societe Belge de Geographie. All rights reserved.

Establishment of angiotensin-converting enzyme 2 and cluster of differentiation 147 dual target cell membrane chromatography based on SNAP-tag technology for screening anti severe acute respiratory syndrome coronavirus 2 active components.

Patients have different responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections and these may be life-threatening for critically ill patients. Screening components that act on host cell receptors, especially multi-receptor components, is challenging. The in-line combination of dual-targeted cell membrane chromatography and a liquid chromatography-mass spectroscopy (LC-MS) system for analyzing angiotensin-converting enzyme 2 (ACE2) and cluster of differentiation 147 (CD147) receptors based on SNAP-tag technology provides a comprehensive solution for screening multiple components in complex samples acting on the two receptors. The selectivity and applicability of the system were validated with encouraging results. Under the optimized conditions, this method was used to screen for antiviral components in Citrus aurantium extracts. The results showed that 25 µmol /L of the active ingredient could inhibit virus entry into cells. Hesperidin, neohesperidin, nobiletin, and tangeretin were identified as antiviral components. In vitro pseudovirus assays and macromolecular cell membrane chromatography further verified the interaction of these four components with host-virus receptors, showing good effects on some or all of the pseudoviruses and host receptors. In conclusion, the in-line dual-targeted cell membrane chromatography LC-MS system developed in this study can be used for the comprehensive screening of antiviral components in complex samples. It also provides new insight into small-molecule drug-receptor and macromolecular-protein-receptor interactions.

Increase of nutritional security in Sub-Saharan Africa through the production of dried products from underutilized crops

Currently, an estimated 20% of the population in Sub-Saharan Africa is food insecure with the incidence of hunger and malnutrition still rising. This trend is amplified by the socio-economic consequences of the COVID-19 pandemic. In contrast, more than a third of the harvestable perishable produce is lost due to a lack of preservation or failure to utilize preservation as is the case for underutilized crops (UCs). Moreover, some of the preservation techniques utilized are poor, leading to the deterioration of food quality, especially the micronutrients. In this study, we thus exemplarily investigated the impact of different drying settings on the quality of two highly nutritious UCs, namely cocoyam and orange-flesh sweet potato (OFSP) (40, 60, and 80 °C for cocoyam and 40, 50, 60, and 70 °C for OFSP) to deduce the optimum quality retention and further develop a theoretical design of processing units and processing guidelines for decentralized food processing. Drying cocoyam at 80 °C and OFSP at 60 °C, respectively resulted in a relatively shorter drying time (135 and 210 min), a lower total color difference (2.29 and 11.49-13.92), greater retentions for total phenolics (0.43 mg GAE/100 gDM and 155.0-186.5 mg GAE/100 gDM), total flavonoid (128 mg catechin/100 gDM and 79.5-81.7 mg catechin/100 gDM) and total antioxidant activity (80.85% RSA and 322.58-334.67 mg AAE/100 gDM), respectively for cocoyam and OFSP. The β-carotene, ascorbic acid and vitamin A activity per 100 gDM of the OFSP flours ranged between 6.91- 9.53 mg, 25.90 − 35.72 mg, and 0.53 − 0.73 mg RAE, respectively. © 2022 The Author(s). Published with license by Taylor and Francis Group, LLC.

Phytochemicals of Citrus Fruits: The in-silico Investigation against Sars-CoV-2 Proteins

The current pandemic Covid-19 brought about by a newly emerged and highly infectious virus named as Sars-CoV-2 as a worldwide danger, has infected more than 600 million people and number of deaths are continuously rising day by day. Till date there are no medications accessible for treatment. All over the world scientists and researchers are involved in the study of this emerged virus and its lifecycle. Structures of proteins in the life cycle of virus has been revealed in RCSB PDB (Research Collaboratory for Structural Bioinformatics Protein Data Bank) by researchers. Citrus fruits are used to treat many distresses of humans. Literature survey shows that it has various activities. Our research work is meant to identify the phytoconstituents which are having phenolic composition and good antiviral and antioxidant properties from citrus fruits against Covid-19 proteins (spike binding domain with ACE2 receptor and spike binding domain with Main protease) and to know its in-silico molecular basis. In this study, about 25 compounds from citrus fruits which is having a good antiviral and antioxidant properties and also phenolic composition were employed for molecular docking analysis, molecular dynamic simulation studies and ADME studies. Based on present study 2 compounds from Citrus fruits acted well against the Covid-19 proteins. The MD simulations were employed to identify Hesperidin and Procyanidin B2 as hit compounds. Further ADME analysis were studied for top 2 compounds, these compounds can be further taken for in-vitro studies to know the effective activity against Covid-19. © 2023, Walailak University. All rights reserved.

Automatic Monitoring and Control System for Different Water Levels in Interconnected Multiple Twin Tanks with Dry Run Protection Using Microprocessor Orange Pi

The proposed system effectively controlled and monitored the water level of the dual tank system with efficient dry-run protection to prevent the motor from burning out and avoid wastage of electricity in case of no water. An Orange Pi was used to test its working and ability to control and monitor a real-Time system as no prior research is done on a dual water tank control system using Orangepi SBC. Due to COVID-19, millions of people's financial condition has worsened, which is why an initiative is taken to use the cheap board for making prototypes and moving towards PLCs after the desired outcomes. This research aims to provide a system at cheaper rates to handle large water tanks. This system is very efficient and valuable in dams, tanks, purifiers, and water containers. The proposed system also has dry run protection to avoid wastage of electricity in case of no water. The research is an advancement in automation in real-Time virtual monitoring and different water level control systems. This research makes life more comfortable because real-Time monitoring with a water control system reduces water wastage, leading to the complete modern solution of the problems. © 2022 IEEE.

FLAVONOIDS AS A STARTING POINT FOR THERAPEUTICS AGAINST COVID-19: CURRENT STATE-OF-THE ART RESEARCH ADVANCES

A large amount of biologically active compounds, including phenolic acids and flavonoids, are present in the citrus fruits’ peel. If these compounds can be fully extracted from waste citrus peels and utilized, this could not only transform them into value-added byproducts and cut the economic costs, but also reduce the environmental problem of their disposal. Flavonoids, which possess anti-cancer, antimicrobial, antioxidant, and anti-allergic properties, have been extensively applied in the pharmaceutical industry and skincare products. They have also been reported to potentially inhibit coronavirus. In this chapter, the properties of flavonoids will be first discussed. The bioavailability of flavonoids will also be covered. Following this, their potential capacity to inhibit COVID-19 will be illustrated. This chapter offers detailed information from recent studies focused on flavonoids and their extraction and widespread application in the food and pharmaceutical fields. As a starting point for therapeutics against COVID-19, the intake of certain amount of citrus fruits or their extracts could contribute to the prevention of COVID-19. © 2021 by Nova Science Publishers, Inc.

Magnetic Iron-Improved Growth, Leaf Chemical Content, Yield, and Fruit Quality of Chinese Mandarin Trees Grown under Soil Salinity Stress.

Chinese mandarin fruits are an inexpensive and rich source of vitamin C. They have potential benefits in treating acute respiratory infections and mitigating inflammation in critical patients with COVID-19. In Egypt, citrus is the most important fruit tree but is sensitive to salinity stress, resulting in poor vegetative tree growth and reductions in productivity and fruit quality. Magnetic iron has emerged as a promising approach in the citrus tree industry, since it improves vegetative growth, yield, and fruit quality and alleviates salinity stress in Chinese mandarin trees grown in soils suffering from high salt stress. This research is aimed at studying the influence of adding magnetic iron (as soil treatment) on tree canopy growth, yield, and fruit quality of 'Chinese' mandarin trees. Therefore, the treatments were as follows: 0, 250, 500, and or 750 g of magnetic iron.tree-1. Our results indicated that all applications of magnetic iron significantly improved tree canopy volume, leaf total chlorophyll, relative water content, yield (kg.tree-1), and the fruit physical and chemical characteristics of Chinese mandarin. In contrast, leaf Na and Cl content, (%), proline, and total phenolic content were decreased by magnetic iron soil treatments. In respect to vegetative growth, our results indicated that adding magnetic iron at the concentration 750 g.tree-1 caused the best values of tree canopy volume. A similar trend was noticed regarding yield. The increase in yield attained was nearly 19%; the best values were obtained when magnetic iron were used at 750 g.tree-1. In conclusion, the application of magnetic iron can lead to improved fruit production and fruit quality of Chinese mandarin trees grown in salinity stress conditions.

Citrus junos Tanaka Peel Extract Ameliorates HDM-Induced Lung Inflammation and Immune Responses In Vivo.

In the wake of the COVID-19 pandemic, lung disorders have become a major health concern for humans. Allergic asthma is the most prevalent form of asthma, and its treatments target the inflammation process. Despite significant developments in the diagnosis and management of allergic asthma, side effects are a major concern. Additionally, its extreme heterogeneity impedes the efficacy of the majority of treatments. Thus, newer, safer therapeutic substances, such as natural products, are desired. Citrus junos Tanaka has traditionally been utilized as an anti-inflammatory, sedative, antipyretic, and antitoxic substance. In this study, the protective effects of Citrus junos Tanaka peel extract (B215) against lung inflammation were examined, and efforts were made to understand the underlying protective mechanism using an HDM-induced lung inflammation murine model. The administration of B215 reduced immune cell infiltration in the lungs, plasma IgE levels, airway resistance, mucus hypersecretions, and cytokine production. These favorable effects alleviated HDM-induced lung inflammation by modulating the NF-κB signaling pathway. Hence, B215 might be a promising functional food to treat lung inflammation without adverse effects.

Significance and importance of fruits in strengthening immune system during COVID-19

The outbreak of COVID-19 in 2019 followed by its new variants till now in 2021, made it more necessary to find more identify effective antiviral agents to be included in daily life to combat SARS-CoV-2 and support vaccines and their effect. Fruits are always considered good for health and many studies are trying to find the solution and different compounds with antiviral properties in fruits. Recently, many in vivo and docking studies found many phytochemical compounds effective against COVID-19. In this review, we tried to collect data from different studies. We found that fruits are most valuable gift with great healing property.

Citrus clementine peels essential oil exhibited anti-SARS-CoV-2 and its modulatory effect against cytokine storm: Evidence from in vitro and in silico studies

SARS CoV-2 gets over more than four million people all over the world. The challenges for developing vaccines in overwhelming pandemic situations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), developing and screening of unique antiviral agents are peremptorily necessitated. In this study, we aimed to identify the chemical constituents of Citrus clementine peel essential oil (CCPEO) and to investigate its activities as anti-SARS-CoV-2 and anti-inflammatory activities. The chemical profile of CCPEO was identified via Gas chromatography-mass spectrometry analysis (GC/MS). The in-vitro cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and the 50% cytotoxic concentration (CC50) of CCPEO was determined. The antiviral effect of citrus clementine extract was determined by plaque reduction assay. A geometry-based molecular docking approach (Patchdock) was performed to create docking modifications that result in good molecular shape complementarity. The antiviral effect of CCPEO was attributed to the downregulation of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) released from Huh7cells, and thus attenuating the SARSCoV-2 infection-associated cytokine storm in severe cases. ©2022 National Information and Documentation Center (NIDOC)